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Abstract Detail

Growth and Vegetative Development

Holladay, Steven [1], Hohenstatt, Mareike [2], Torres, Mirayda [1], Kolukisaoglu, Uener [3], Wanke, Dierk [4], Lafos, Marcel [5], Schulz, Burkhard [1].

Arabidopsis TWISTED DWARF1 (TWD1) as a model system for “functional phylogeny” studies.

Immunophilins are receptors for immunosupressants which block T-cell receptor mediated signal transduction in mammals. These proteins are ubiquitous in all organisms from bacteria to animals and plants. Plants have a great number of immunophilins but no immune system, what is their role in plants?
Plant immunophilins are found in multiple developmental processes. The membrane-bound FK506-binding (FKBP) immunophilin, TWISTED DWARF1 (TWD1) regulates the polar export of auxin by ABC transporters PGP1 and PGP19. Knock-outs of TWD1 in Arabidopsis result in dwarfism and disorientated growth. Is regulation of polar auxin transport a common function for TWD1 in plants? A phylogenetic approach of cross-species complementation of knock-out mutants will be used to determine the function of TWD1 orthologs. The goal is to elucidate the role of TWD1 in plant development.
Arabidopsis twd1 mutants are a test system for functional complementation by transformation with TWD1 orthologs from other species. Depending on amino acid (aa) alterations acquired during evolution, a more or less complete functional complementation of twd1 phenotype should be visible. Sequence comparisons of the different proteins will reveal which aa positions are altered and which alterations are essential for complete complementation. Likewise, aa alterations without effects will also be identified. Together with protein structure information this approach should give us insight into the function of an immunophilin in its cellular context at very detailed level. TWD1 homologs from dicots tomato and C. rubella have been successfully used as proof of concept.
Here we report on cloning and complementation/transformation of TWD1 homologs from the monocots rice and corn in twd1 of Arabidopsis.

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1 - Purdue University, Horticulture, Horticulture Building, Room 113, 625 Agriculture Mall Drive, West Lafayette, Indiana, 47907-2010, USA
2 - University of Tuebingen, Germany
3 - University of Rostock, Germany
4 - University of Tuebingen, ZMBP, Germany
5 - University of Duesseldorf, Germany

Twisted Dwarf
Plant Immunophilins

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P26033
Abstract ID:905

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