Systematics Section / ASPT
Borrone, James W. , Meerow, Alan W. , Kuhn, David N. , Schnell, Raymond J. , Whitlock, Barbara A. .
The potential of the WRKY gene family for phylogenetic reconstruction: an example from the Malvaceae.
The WRKY gene family of transcription factors is involved in several diverse pathways and are components of plant-specific, ancient regulatory networks. WRKY genes contain one or two highly conserved DNA binding domains interrupted by an intron. We used partial sequences of five independent WRKY loci, originally isolated from Theobroma cacao by PCR with a single pair of degenerate primers, to assess their potential for phylogeny reconstruction. We tested those loci across the sister genera Herrania and Theobroma, with Guazuma ulmifolia as outgroup. All loci-specific primer pairs amplifed a single product that was direct-sequenced. Overall, the combined WRKY matrices performed as well or better than other genes in resolving the intrageneric phylogeny of Herrania and Theobroma. The ease of isolating numerous, independent WRKY loci from diverse plant species with a single pair of degenerate primers designed to the highly conserved WRKY domain, renders them extremely useful tools for generating multiple, single- or low-copy nuclear loci for molecular phylogenetic studies at various taxonomic levels.
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1 - U. S. Dept. of Agriculture, Agricultural Research Service, National Germplasm Repository, 13601 Old Cutler Rd., Homestead, FL, 33158, United States
2 - U. S. Dept. of Agriculture, Agricultural Research Service, National Germplasm Repository, 13601 Old Cutler Rd., Miami, FL, 33158, United States
3 - University of Miami, Department of Biology, 1301 Memorial Drive, Coral Gables, Florida, 33124, USA
Low-copy Nuclear Markers
Presentation Type: Poster:Posters for Sections
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM