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Abstract Detail


Luesse, Darron [1], Justus, Betsy [1], Wyatt, Sarah [2].

Identification of Interacting Partners for the SNARE GPS2.

Virtually all aspects of plant form and development are impacted by gravity. However, very little is known about the signal transduction events that lead to an alteration in growth patterns after a change in the gravity vector. To identify mutants in this portion of the pathway, a screen was conducted to isolate Arabidopsis lines that showed aberrant gravitropism in the inflorescence in response to a reorientation at 4ºC (Wyatt et al. 2002). One mutant isolated in this screen, gravity persistent signal 2 (gps2), bends in the opposite direction of wild type when gravistimulated in the cold. GPS2 was determined to belong to a group of soluble N-ethylmaleimide-sensitive factor adaptor protein receptors (SNAREs) that are involved in vesicle docking. To gain understanding of GPS2s role in gravitropism, we are performing a Bi-Molecular Fluorescence Complementation (BMFC) assay to determine interacting partners for GPS2. In this system, the N-terminal half of YFP is fused with full length GPS2 (N-YFP::GPS2) and expressed constitutively in BY-2 cells. A cDNA library fused with the C-terminal half of YFP (C-YFP) is being screened by particle introducing bombardment into BY-2 cells expressing N-YFP::GPS2. If a member of the cDNA library interacts physically with GPS2, the two halves of YFP are brought into close contact and fluorescence results. Use of this system should allow isolation of GPS2-interacting proteins and provide insight into the signal transduction pathways required for gravitropic curvature.

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1 - Ohio University, Environmental and Plant Biology, 512 Porter Hall, Athens, OH, 45701, United States
2 - Ohio University, Department of Environmental & Plant Biology, Porter Hall, Richland Avenue, Athens, Ohio, 45701-2979, USA

Signal transduction.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P32003
Abstract ID:878

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