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Abstract Detail


Growth and Vegetative Development

Nugent, Anna [1], Mohamed, Abeer [2], Short, Timothy [2].

Stable Transformation of Ceratopteris richardii.

The fern Ceratopteris richardii has been a useful model system for studies of cell biology and genetics. A significant weakness of this model system has been the lack of a reliable transformation system. In an effort to achieve transgene expression and transformation in Ceratopteris richardii, constructs harboring 35S:GFP and actin:GFP were microinjected or bombarded into young prothalli. Using these methods, transient expression was observed, with the 35S promoter driving the more vital and efficient expression. Stable transformation is now being attempted using an Ac/Ds transposon system, with the 35S promoter driving a YFP gene in the Ds element. The Ds element and the transposase are co-bombarded on separate plasmids into newly germinating spores. This system will be used to examine proteins involved in photomorphogenesis as well as broader developmental questions in ferns. Initial results of stable transformation will be reported.


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1 - Queens College & The CUNY Graduate Center, Biology, 65-30 Kissena Blvd, Flushing, New York, 11367, USA
2 - Queens College & The CUNY Graduate Center, Biology

Keywords:
ferns
transformation.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P26032
Abstract ID:869


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