| Abstract Detail
Environmental Physiology Sanjaya, Sanjaya [1], You, Su-Juan [2], Chan, Ming-Tsair [2]. Overexpression of Arabidopsis ZAT10 Gene Enhances Salt Tolerance in Transgenic Tomato Plants. Among the adverse environmental factors commonly encountered by land plants are extremes in temperature and osmotic stress, which can result in either water deficit or salinity stress. The STZ/ZAT10 is belonging to Cys2/His2-type zinc-finger proteins, identified during screening of Arabidopsis genes that complement the salt-sensitive phenotype of a yeast mutant which is deficient in the phosphoprotein phosphatase, calcineurin and is involved in salt tolerance of Arabidopsis. Tomato transgenics overexpressing Arabidopsis ZAT10 gene were developed in Taiwan popular variety (CL591-93D4-1-0-3) via Agrobacterium-mediated transformation using the protocol developed previously. Modified binary vector pBI221 harboring ZAT10 gene driven by 2X cauliflower mosaic virus (CaMV) 35S promoter and hptII gene was used. The integration and expression of foreign gene in the putative transgenic plants were confirmed by molecular analysis. A total of 20 independent T0 putative transgenic lines were developed, T1 plants were obtained from T0 seeds. The ZAT10 function in vivo is demonstrated in tomato plants under the control of constitutive promoter show a significant increased salt-stress tolerance (200 mM) than wild-type plants. Further investigations at molecular level are in progress to elucidate the role of ZAT10 in salt signal transduction pathway in tomato.
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1 - Agricultural Biotechnological Research Center, Academia Sinica, A304, 3rd floor, Institute of chemistry, Academia Sinica, Nankang, Taipei, 11529, Taiwan
2 - Agricultural Biotechnology Research Center, Academia Sinica
Keywords:
ZAT10
tomato
genetic engineering
salt stress
crop.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P01021
Abstract ID:815 |
