Abstract Detail
Stress Tolerance Husaini, Amjad Masood [1], Abdin, Malik Zainul [1]. Overexpression of tobacco osmotin gene leads to salt stress tolerance in strawberry (Fragaria x ananassa Duch.) plants. Transgenic plants of strawberry tolerant to salt stress were produced using Agrobacterium mediated gene transfer technology. Leaf discs of in vitro grown plantlets of strawberry (cv. chandler) were used as explants. Agrobacterium tumefaciens strain GV2260 harbouring osmotin gene under the control of CaMV 35 S promoter in a binary vector system (pBinAR) was used in co-cultivation experiments. Transgene integration and copy number were assessed by PCR and Southern hybridization confirming single copy as well as multiple copies of transgene integration in 10 different lines of transgenic strawberry. Expression of osmotin gene was confirmed in transgenic lines TL3, TL5, TL9 using Northern hybridization, while biochemical analyses of these transgenic plants revealed enhanced levels of proline, total soluble protein and chlorophyll content as compared to the wild plants. Leaf disc assays performed using both wild type and transgenic plants had shown that these transgenic lines were tolerant to salt stress.
Log in to add this item to your schedule
1 - Jamia Hamdard University, Department of Biotechnology, Hamdard Nagar, New Delhi, Delhi, 110062, India
Keywords:
Agrobacterium
transgenic
salt stress
strawberry
in vitro.
Presentation Type: Poster:Posters for Topics
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P77003
Abstract ID:79 | 