Guerrero, Noemi , Shi, Huazhong .
Identification of Genetic Loci Important for the Gene Regulation of AtERF3.
Plants are sessile and therefore have to cope with different environmental stresses. One such stress is salinity, which affects crop production world-widely. Many genes important for stress tolerance have been cloned in different plant species. These genes could be of high significance in agriculture because they could be used to genetically improve stress tolerance in crops. Ethylene responsive element binding factors (ERFs) are part of a family of transcription factors that have only been found in higher plants. In Arabidopsis, one of the ERF gene families, AtERF3, is moderately induced by salinity and drought stress. To identify the signaling components controlling AtERF3 gene expression, the promoter of AtERF3 was fused with a firefly luciferase reporter gene (AtERF3P-LUC). Homologous lines of transgenic plants harboring the chimeric gene construct were generated. Seeds of one homologous line were subjected to EMS-mutagenesis. The M2 seeds were used for forward-genetic mutant screening by using a high sensitive, low light CCD imaging system capable of monitoring the luciferase expression level. We have screened approximately 150,000 individual plants and identified several hundreds of putative mutants showing higher or lower luciferase expression. Confirmation of these mutants by re-screening is in progress. Our research will identify both negative and positive regulators controlling AtERF3 gene expression.
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1 - Texas Tech University, Chemistry and Biochemistry, Box 41061, Lubbock, Texas, 79409-1061, USA
2 - Texas Tech University, Chemistry and Biochemistry
CCD imaging system.
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM