Kalampanayil, Bose , Wimmers, Larry , Bradley, Brian .
Proteomic analysis of drought stress-responsive proteins in tomato.
Drought, defined as the occurrence of a substantial water deficit in the soil is an increasingly important constraint to crop productivity. Majority of crop plants, including tomato are sensitive to drought stress throughout the ontogeny of the plant, from seed germination to harvest. An approach to minimizing agricultural losses incurred by drought stress is to develop plant cultivars that can escape or withstand periods of drought. The overall goal of this project was to identify proteins involved in response to drought stress in tomatoes. Combined proteomic approaches, two dimensional gel electrophoresis (2 DE) and mass spectrometry, revealed the plasticity of gene expression by protein modification during environmental stress. Tomato plants were grown hydroponically in aerated nutrient solution and polyethylene glycol was increased daily in increments to accomplish final concentrations on the same day. Roots and mature leaves were collected at day 1 and 5 post-treatments, and these tissues were subjected to two-dimensional gel electrophoresis. A computer image analysis software program, PDQuest (Bio-Rad) was applied to the 2 DE gels for detection of stained proteins, background subtraction and signal matching between treated and control samples. Following completion of the PDQuest analysis, specifically repressed and induced proteins were identified from control and treated tissues using liquid chromatography mass spectrometry. These proteins include various transcription factors, transporters, enzymes involved in solute accumulation and water homeostasis, and stress tolerance proteins.
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1 - University of Maryland, Baltimore County, Department of Biological Sciences
2 - Towson University, Department of Biological Sciences, 8000 York Rd, Towson, MD, 21128, USA
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM