| Abstract Detail
Ranunculales - a Basal Eudicot Perspective of Angiosperm Evolution Lange, Matthias [1], Wege, Stefanie [1], Orashakova, Svetlana [1], Becker, Annette [2]. Evolutionary developmental genetics of carpels in the basal eudicot model species California poppy. The female reproductive organs of angiosperm flowers, the carpels, have undergone enormous morphological diversification in their more than 125 million years of evolution. We are interested in the genetically based developmental processes leading to the morphologically conserved and diverse aspects of carpels, which are to a large extent controlled by transcription factors (TF) of diverse families.
We therefore functionally and structurally investigate important transcription factors and their cis-regulatory elements most likely involved in carpel development of our Ranunculales model plant California poppy Eschscholzia californica. This species is genetically tractable and studies are underway to determine the expression pattern and functions of carpel genes with respect to comparable data from Arabidopsis and rice orthologs. Knock-down of the Arabidopsis ortholog of CRABS CLAW (CRC) in poppies (EcCRC) by Virus-induced gene silencing (VIGS) shows reduced floral determinacy, indicating a different function which contrasts to its role in Arabidopsis, where CRC is involved in nectary and gynoecium development and only to a much lesser extent in floral determinacy. Expression studies as well as functional studies using VIGS and stable genetic transformation are being carried out with poppy orthologs of several Arabidopsis and rice carpel development genes. We also have constructed a Bacterial Artificial Chromosome (BAC) library to obtain cis-regulatory sequences of carpel development controlling TF in order to explain differences in their expression pattern due to structural cues in these sequences. The integration of functional data from diverse angiosperms will lead to a better understanding of the conserved core of developmental regulators of floral architecture and how the underlying regulatory networks have evolved.
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1 - University of Bremen, Evolutionary Developmental Genetics Group, Leobener Strasse, UFT, Bremen, D-28359, Germany
2 - University Bremen, Evolutionary Developmental Genetics Group, Leobenerstrasse UFT, Bremen, D-28359, Germany
Keywords:
Eschscholzia
Carpel development
Ranunculales
EcCRC
Virus-induced gene silencing
promotor evolution.
Presentation Type: Symposium or Colloquium Presentation
Session: SY19
Location: Stevens 2/Hilton
Date: Wednesday, July 11th, 2007
Time: 2:15 PM
Number: SY19003
Abstract ID:759 |
