Regulation of Metabolism
Huang, Yadong , Li, Chunyao , Gibson, Susan .
Identification of a Protein Kinase and a RING-Finger Protein Involved in Plant Metabolic Response.
In plants, metabolism and development are tightly integrated. For example, the levels of soluble sugars, such as glucose and sucrose, regulate diverse metabolic and developmental pathways. To elucidate the role of soluble sugars in plant metabolism and development, our lab has identified and characterized several sugar insensitive (sis) mutants of Arabidopsis. These sis mutants are resistant to the inhibitory effects of high concentrations of sucrose and glucose on early seedling development. The SIS7 gene was cloned using a map-based approach and found to be identical to the previously identified STO1/NCED3 gene. This gene encodes 9-cis-epoxycarotenoid dioxygenase, a key enzyme in the biosynthesis of abscisic acid. In addition to being resistant to sucrose and glucose, the sis7-1/sto1-2/nced3-2 mutant exhibits resistance to mannose and to the gibberellin biosynthesis inhibitor paclobutrazol during seed germination. Genome-wide transcriptional analysis of sis7-1/sto1-2/nced3-2 reveals that this mutation affects the steady-state mRNA levels of a diverse array of genes during seed germination. Recently the genes corresponding to two other sis mutants, sis3 and sis8, were identified by map-based cloning. The SIS3 gene encodes a protein that is predicted to be a member of the RING-finger family. The SIS8 gene is predicted to encode a protein kinase. Current efforts are focused on characterizing SIS3 and SIS8, including using biochemical approaches to characterize protein activity, expressing fusion proteins to examine protein localization and using the yeast two-hybrid technique to screen for interacting proteins.
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1 - University of Minnesota, Plant Biology, 1500 Gortner Ave., Cargill Bldg. Room 350, St. Paul, MN, 55108, USA
2 - University of Minnesota, Plant Biology
Presentation Type: ASPB Minisymposium
Location: Continental A/Hilton
Date: Wednesday, July 11th, 2007
Time: 11:05 AM