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Abstract Detail

Reproductive Development

Xu, Xianfeng, Morgan [1], Rose, Annkatrin [2], Muthuswamy, Sivaramakrishnan [1], Jeong, Sun Yong [3], Venkatakrishnan, Sowmya [1], Zhao, Qiao [1], Meier, Iris [1].

NUCLEAR PORE ANCHOR, the Arabidopsis homolog of Tpr/Mlp1/Mlp2/Megator, is involved in mRNA export and SUMO homeostasis and affects diverse aspects of plant development.

Vertebrate Tpr and its yeast homologs Mlp1/Mlp2, long coiled-coil proteins of nuclear pore inner basket filaments, are involved in mRNA export, telomere organization, spindle pole assembly, and unspliced RNA retention. We identified Arabidopsis NUCLEAR PORE ANCHOR (NUA) encoding a 237 kD protein with similarity to Tpr. NUA is located at the inner surface of the nuclear envelope in interphase and in the vicinity of the spindle in prometaphase. Four T-DNA insertion lines were characterized, which comprise an allelic series of increasing severity for several correlating phenotypes, such as early flowering under short days and long days, increased abundance of SUMO conjugates, altered expression of several flowering regulators, and nuclear accumulation of poly(A)+ RNA. Nua mutants phenocopy mutants of EARLY IN SHORT DAYS4 (ESD4), an Arabidopsis SUMO protease concentrated at the nuclear periphery. nua esd4 double mutants resemble nua and esd4 single mutants, suggesting that the two proteins act in the same pathway or complex, supported by yeast two-hybrid interaction. Our data indicate that NUA is a component of nuclear pore-associated steps of sumoylation and mRNA export in plants and that defects in these processes affect the signaling events of flowering time regulation and additional developmental processes.

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1 - The Ohio State University, Plant Cellular and Molecular Biology, 244 Rightmire Hall, 1060 Carmack Rd., Columbus, OH, 43210, USA
2 - Appalachian State University, Department of Biology
3 - Unversity of North Carolina at Chepel Hill, Department of Biochemistry and Biophysics

Nuclear Envelope
Nuclear Pore
mRNA export

Presentation Type: ASPB Minisymposium
Session: M18
Location: International Ballroom/Hilton
Date: Tuesday, July 10th, 2007
Time: 3:45 PM
Number: M18002
Abstract ID:682

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