Robertson, Amber L. , Rao, Hongyu , Wang, Wuyi , Patterson, Sara E. .
Secretory Carrier Membrane Proteins (SCAMPs) in Arabidopsis thaliana.
Secretory Carrier Membrane Proteins (SCAMPs) are found ubiquitously in eukaryotic cells and share a high degree of homology across taxa. All recognized SCAMPs share nine highly conserved protein domains including four transmembrane domains and a NPF domain (Hubbard et al., 2000). SCAMPs have been implicated in processes as varied as neuron trafficking, endocytosis, and floral organ abscission (Brand et al., 1991, Fernandez-Chacon and Sudhof, 2000, and Patterson et al., submitted). We have identified a mutant SCAMP, dab 5-1, in a screen for delayed floral organ abscission. Expression studies using both RT-PCR and plants transformed with a DAB5 promoter::GUS fusion construct indicate constitutive expression of AtSCAMP5. Confocal microscopy of plants expressing DAB5:DAB5-GFP shows localization to the plasma membrane and small post-Golgi endocytic vesicles in agreement with localization studies performed in animal cells (Castle and Castle, 2005 and Lam, 2007). Given the changes in the phenotype of this single SCAMP mutant, we wanted to further investigate SCAMPs in plants to determine their function and thus better understand the processes regulating cell separation. We are characterizing the five Arabidopsis SCAMPs that are 88% identical to each other. In addition to investigating the changes in phenotype of each single mutant, we are also analyzing the effect of multiple mutations on plant development. Changes in the expression pattern of individual SCAMPs in different the genetic backgrounds and preliminary information on protein-protein interactions will also be presented.
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1 - University of Wisconsin- Madison, Horticulture, 1575 Linden Dr., Madison, WI, 53706, USA
2 - University of Wisconsin- Madison, Horticulture
3 - Ceres, Inc.
Presentation Type: ASPB Minisymposium
Location: Continental B/Hilton
Date: Monday, July 9th, 2007
Time: 8:30 AM