| Abstract Detail
Vegetative Development Sena, Giovanni [1], Wang, Xiaoning [2], Birnbaum, Kenneth [3]. A Genomic and Real Time View of Regenerating Tissue. One example of a self organizing system is the regeneration of lost or damaged tissue in post embryonic plant and animal development. We have designed an experimental system in plants in which we can track morphological and molecular events during the regeneration of root tips using confocal microscopy, time series microarray profiles, and mutant analysis. Using analytical methods that classify transcriptional states of cells at global level, we use dynamic expression profiling to map the trajectory of regenerating cells onto a landscape of cell specific transcriptional signatures. We show the rapid recovery of cell-specific gene networks within hours of root tip excision. We also show that early transcriptional trajectory of regenerating tips have strong affinities to meristematic tissue from undamaged roots but not to stem cells. Time lapse confocal microscropy of the stem cell niche during regeneration support the systems view that recovery of excised cell types occurs before the reformation of the stem cell niche. In addition, we show that mutants lacking a functional stem cell niche are able to regenerate. Thus, self organization of the excised root tip proceeds in an environment in which classical stem function is not present. Regenerative competence maps to a larger set of cells than those of the classical stem cell niche.
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1 - New York University, Rockefeller University, Biology
2 - New York University, Biology
3 - New York University, Biology, New York, NY, 10003, USA
Keywords:
genomics
regeneration
developmental imaging.
Presentation Type: ASPB Minisymposium
Session: M02
Location: Continental B/Hilton
Date: Sunday, July 8th, 2007
Time: 2:00 PM
Number: M02001
Abstract ID:612 |
