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Abstract Detail

Phycological Section

Agrupis, Shirley C. [1], Azuma, Jun-ichi [2].

Important Algal Polysaccharides Extracted from Undaria undarioides(Yendo) Okamura.

Two important polysaccharides; fucoidan and alginate, were successfully extracted from brown algae, Undaria undarioides. This kelp, locally known as Hirome in Japan, productively thrive in the warm waters in Wakayama and Okinawa Prefecture. Fucoidan and alginate are complex polysaccharides found in the cell wall matrix of brown algae and has recently gained prominence because of their wide range of industrial and biomedical applications. Fucoidan has a number of advancements in cancer treatment and other biomedical uses while alginate is widely used as gelling agent and stabilizers for foods and recently been explored for biological and biotechnological applications. Sugar content of fucoidan analyzed by HPAEC with sulfuric acid hydrolysis showed galactose (47.29%) and fucose (37.68 %) as its primary component sugars while Fucose (37.01%) dominates in alginate. Mannose, xylose, and glucose were present in varied concentrations. The infrared absorption properties of the isolated fucoidan and alginate were consistent with the previous studies from other brown algae sources. The spectrum of fucoidan indicated a hydroxyl group (H-O stretching) at 3422 cm-1. The prominent band at 1254 cm-1 was caused by the S=O stretching vibrations. The band at 820-850 (C-O-S) cm-1 is common to all sulfated polysaccharide due to sulfate absorption. The desired signals of the two polysacharrides were not detected in the prepared samples for the 13C-NMR analysis due to their high viscosity that hampered good peak resolution. For fucoidan, which gave better resolution than alginate, the weak signals at 5.34 ppm and 1.27 ppm indicated the presence of L-fucopyranosyl units and methyl protons of the L-fucosyl residues. Also, the prominent signal at 2.17 ppm indicated the methyl signal of the acetyl group of the fucoidan structure. The above results indicate the great promise of this algal species for further exploration to varied biomedical and industrial applications.

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1 - Mariano Marcos State University (MMSU), Biology, Room 201, Admin Building, MMSU, Paoay Road, Batac, Ilocos Norte, 2906, PHILIPPINES
2 - Kyoto University, Graduate School of Agriculture, Laboratory of Forest Biochemistry, Kita-shirakawa, Oiwake-cho, Sakyo-ku, Kyoto-shi, 606-8502, JAPAN

Brown algae.

Presentation Type: Oral Paper:Papers for Sections
Session: CP35
Location: Lake Michigan/Hilton
Date: Tuesday, July 10th, 2007
Time: 4:00 PM
Number: CP35001
Abstract ID:593

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