| Abstract Detail
Metabolism Choi, Suh-Yeon [1], Ilarslan, Hilal [2], Kourtz, Lauralynn [3], Snell, Kristi [3], Lawrence, Michael [4], Cook, Dianne [4], Nikolau, Basil J [5], Wurtele, Eve [2]. Transcriptomic and Metabolomic analysis of PHB-producing Arabidopsis. Polyhydroxyalkanoates (PHAs) are a class of biodegradable polyesters (bioplastics) naturally produced by many bacteria. Production of PHAs in plants could provide a solar-energy-powered source of bioplastic. However, economically viable production of bioplastics in plants tends to have detrimental effects on growth, which limits the use of plants as bio-factories. To understand the relationship between carbon utilization and illumination cycles in the presence of a foreign sink for plastidic acetyl-CoA, we evaluated transgenic PHB-producing and wild type plants grown under three different illumination regimes. PHB accumulation was dependent on the light regime, and was as high as 15% of total dry weight. Metabolite profiles (non-targeted GC-MS analysis), the acyl-CoAs accumulation (LC/MS/MS), and transcriptomic profiles were determined. Transcriptomic and metabolomic data were evaluated using Explorase (MetNet Platform, http://metnet.vrac.iastate.edu) software to analyze and visualize omics data. Our working hypothesis is that that plant responds to a flux of carbon to PHB with transcriptional shifts that reallocate carbon in related pathways. Log in to add this item to your schedule
Related Links: MetNet platform to analyze and visualize omics data
1 - Iowa State University, Genetics, Development and Cell Biology, 433 Bessey Hall, Iowa State University, Ames, IA, 50011, USA 2 - Iowa State University, Genetics, Development and Cell Biology 3 - Metabolix Inc. 4 - Iowa State University, Statistics 5 - Iowa State University, Biochemistry, Biophysics, and Molecular Biology
Keywords: polyhydroxybutyrate bioplastics Acetyl-CoA metabolomics.
Presentation Type: Plant Biology Abstract Session: P Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton Date: Sunday, July 8th, 2007 Time: 8:00 AM Number: P19022 Abstract ID:534 |