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Plant biotech & Risk Assessment

Sharma, R. K. [1], Negi, M. S. [2], Bhardwaj, P. [3], Sehagal, D. [4], Negi, R. [3], Tewari, M. [5], Tripathi, S. B. [2], Banik, J. [5], Karthigeyan, S. [6], Vijayan, D. [7], Chowdhury, B. R. [8], Bera, B. [9], Das, S. C. [10], RajKumar, R. [7], Mandal, A.K.A [11], Sen Mandi, S. [8], Murleedharan, N. [11], Hazarika, M. [12], Raina, S. N. [5], Ahuja, P. S. [13].

AFLP marker-assisted rationalization of Indian tea germplasm collections.

India is one of the world's largest producers and consumer of tea and also a custodian of the diverse germplasm. Tea, Camellia germplasm is comprised of accessions and hybrids amongst the China (C. chinensis), Assam (C. assamica) and Cambod (C. assamica ssp lasiocalyx) types. Therefore, there is a need of characterization of the swarm of hybrids that are highly heterogeneous at the molecular level for its proper management and to identify redundant germplasm so as to establish core collections. North eastern (NE), north western (NW) and southern (S) geographical regions are the source of > 90% tea production in India. In the present study ~2000 tea accessions available at Tea Research Association, Tocklai, Jorhat, Assam (NE), UPASI Tea Research Foundation, Valparai, Tamilnadu (Southern) and Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh (NW), and representing the total spectrum of variation of the cultivated teas (landraces, core collections and garden selections) in India were used to study polymorphism comprehensively at the DNA sequence level. So far, a total of 1420 cultivated Indian tea clones have been characterized by 10 AFLP primer combinations at the national level in a coordinated manner at six Institutes. Preliminary data analysis of 940 tea clones has accounted for only 7% redundancy. This study revealed that 483 (> 51 %) tested tea germplasm have recorded 90 % genetic similarity. Of the analyzed 940 tea accessions approximately 236 (>25 %) tea accessions grouped into three major clusters have registered considerable genetic diversity and therefore can be considered as the core collections from amongst these clones. This is now being extrapolated on the basis of analysis of 2000 tea accessions.


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Related Links:
IHBT
Tea Research Association
Upasi Tea Research Foundation


1 - Institute of Himalayan Bioresource Technology (IHBT), Biotechnology, Post Box -6, Palampur, HP, +91, India
2 - TERI, PMB
3 - IHBT, Biotechnology
4 - DU, Botaby
5 - DU, Botany
6 - IHBT, Breeding
7 - UPASI, Biotech
8 - Bose Institute, Kolkotta, Department of Botany
9 - TRA, Biotech
10 - TRA, Breeding
11 - UPASI, Biotech & Physiology
12 - TRA, Biotech & Physiology
13 - Institute of Himalayan Bioresource Technology (IHBT), Biotechnology

Keywords:
AFLP
Camellia sinensis
Genetic diversity
Tea accessions.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P45005
Abstract ID:418


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