| Abstract Detail
Mineral Nutrition Lee, Sichul [1], An, Gynheung [2]. Alteration of Iron Use efficiency by yellow stripe1- like (OsYSL) genes in rice. The uptake and translocation of metal nutrients are essential for plant growth. Graminaceous plants release ferric chelating phytosiderophores that bind to Fe and then Fe-phytosiderophore complexes across plasma membrane by YELLOW STRIPE 1 (YS1). Here, we report the characterization of the eighteen rice YSL (yellow stripe1-like, designated OsYSL) genes by examining their expression patterns under the micronutrient deficiency and in various organs. To study functional roles of the genes, we have identified two independent activation tagged mutants in OsYSL16 from the pool of rice T-DNA insertional lines transformed with activation tagging vectors. The phenotype of OsYSL16 activation mutants was tested by growing the plants on iron-deficient medium. Visual differences were reflected by the chlorophyll contents, indicating that the OsYSL16-activated mutants are more tolerant to iron deficiency compared to the wild type. Iron content of the OsYSL16-activated line in shoots is higher than that of wild type. The rate of utilization of limited concentration of iron from the seeds was higher in activation tagging mutants than in wild type. Promoter fusions of OsYSL16 to GUS showed that OsYSL16 preferentially expressed in vascular tissues. We have also characterized OsYSL15, one of the OsYSL genes that are strongly induced by the iron deficiency. Two insertional osysl15 mutants showed the chlorotic phenotypes under iron deficiency and reduced iron contents in shoots, root and seeds. Nitric oxide could revert the chloritic phenotypes of the mutant plants growing on the iron deficiency. All together, these results support the biological roles of OsYSL in uptake and long-distance transport of iron.
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1 - Pohang University of Science and Technology, Life Sciences, san31, Hyo-ja Dong, Nam gu, Pohang, Kyungbuk, 790-784, korea
2 - Pohang University of Science and Technology, Life Sciences
Keywords:
rice iron nutrition
OsYSL.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P11005
Abstract ID:374 |
