Abstract Detail
Plant-Pathogen Interactions Wang, Lin [1], Glazebrook, Jane [2]. Two members of the AtCBP60 protein family are required for Arabidopsis defense responses against Pseudomonas syringae. Plant defense against bacterial pathogens is a complex and sophisticated system under the control of numerous genes. Understanding how these genes function and how signals are transduced in the disease signaling network will be potentially beneficial to enhancing disease resistance in crop species. Our lab has developed a high-throughput method for studying functions of genes that are involved in Arabidopsis defense responses against Pseudomonas syringae. Genes that were significantly up-regulated after P. syringae infection were chosen as our candidates based on microarray analysis. Mutants with defects in these target genes were then studied to determine whether they show enhanced disease susceptibility (eds) phenotypes. Genes disrupted in confirmed eds mutants are subjected to further functional studies, which include microarray profiling experiments designed to reveal their roles in disease signaling and studies that lead to understanding of their detailed biological functions.
Currently my research is focusing on a gene family encoding calmodulin binding like proteins, of which several knock-out mutants have shown consistent eds phenotypes. Profiling experiments based on our custom microarray chip indicates mutations in one member of this gene family affect a subset of genes under the regulation of PAD4, suggesting it may function downstream of PAD4 in disease signaling. Calmodulin binding analyses have shown one member of this family encodes a bona-fide calmodulin binding protein, and its calmodulin domain(s) are being investigated for their importance in disease response. A yeast two hybrid screen for interacting proteins is underway, and I am making various transgenic plants expressing fusion proteins to determine the subcellular localization of these proteins. Progress in these areas will be reported at the conference.
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Related Links:
Functional Genomics of Arabidopsis Disease
1 - University of Minnesota, Plant Biology, 350 Cargill Building, 1500 Gortner Ave, St Paul, MN, 55108, United States
2 - University of Minnesota, Plant Biology
Keywords:
bacterial defense
Pseudomonas syringae.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P15023
Abstract ID:339 | 