| Abstract Detail
Secondary Metabolism Vibhu, Bakshi [1], Maier, Camelia [2], Smith, Don W. [3]. Establishing In vitro Cultures of Moraceae Species for the Production of Phytoestrogens. Comparison of phytoestrogens of Moraceae species from in vivo and in vitro tissue isolation. Tissue cultures of mulberry species and Osage-orange, Maclura pomifera (Moraceae), species known to contain phytoestrogens, were established on plant-hormone supplemented Murashige and Skoog (MS) media. In vitro-obtained seedlings tissues, such as epicotyl, hypocotyl and petiole and the stems and petioles from mature trees were the best explants for initiation and proliferation of calli. Callus proliferation was obtained on MS medium containing NAA (1mg/ml) and BAP (0.5mg/ml) for mulberry and IBA(0.5mg/ml) and BAP (0.4mg/ml) for M. pomifera. The estrogenic activities of callus extracts were assayed in an estrogen-responsive yeast system expressing the human estrogen receptor alpha. Male M. alba and female M. rubra callus extracts showed the highest estrogenic activities. Isolation and characterization of phytoestrogens from above callus extracts were carried out using SPE and HPLC chromatographic techniques. HPLC profiles of callus extracts were different from those of mature tree extracts. This indicates that phytoestrogens in in vitro cultures are different than those isolated from the corresponding mature tissues.
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1 - University of North Texas, Biological Sciences, P O Box 305220, Denton, Texas, 76203-5220, USA
2 - Texas Woman's University, Biology
3 - University of North Texas, Biological Sciences
Keywords:
phytoestrogens
tissue culture.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P20018
Abstract ID:336 |
