| Abstract Detail
Emerging Technologies Cai, Charles [1], Baker, Lisa [2], Urnov, Fyodor [3], Miller, Jeffrey [3], Worden, Andrew [4], Blue, Ryan [4], Bauer, Teresa [4], Gregory, Philip [3], Petolino, Joseph [4]. Gene Targeting in Plants via Engineered Zinc Finger Proteins. Gene targeting via homologous recombination occurs at a very low frequency in plant cells compared to random integration making targeted gene modifications impractical. Most recently, substantial increases in the frequency of homologous recombination have been observed following the induction of double stranded breaks in host cell DNA followed by the apparent stimulation of cellular repair mechanisms. Strategies to achieve targeted DNA double stranded breaks have been developed by fusing zinc finger DNA binding proteins with sequence-independent nuclease domains derived from Type IIS restriction endonucleases. Using this strategy, site-specific transgene integration has been demonstrated in plants as has targeted modification of specific genes. Implications for novel trait development and crop improvement will be discussed.
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1 - Dow AgroSciences, LLC, Biochemistry and Molecular Biology/Discovery, 306/1A-2108, 9330 Zionsville Rd., Indianapolis, Indiana, 46268, United States of America
2 - Dow AgroSciences, LLC, Cell Biology/Discovery
3 - Sangamo BioSciences, Inc., Richmond, California, USA
4 - Dow AgroSciences, LLC, Biochemistry and Molecular Biology/Discovery
Keywords:
Gene targeting
homologous recombination
zinc finger nucleases
plant cell.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P44006
Abstract ID:335 |
