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Abstract Detail


Emerging Technologies

Katam, Ramesh [1], Vasanthaiah, Hemanth KN [2], Basha, Sheikh M [2], Frank, Matta [3].

Proteomic and Transcript Analysis of Grapevine Responses to Water Stress.

Water deficit stress has major impact on grape production and quality. However, the desirable outcome of water stress is an improvement in wine quality by increase in the nutrceutical and enological traits of berries. Our primary goal is to identify genes and proteins differentially expressed in response to induced water stress in Florida hybrid bunch grape. Investigating transcripts and proteins will allow us to correlate them and confirm the involvement of specific genes responding to stress. We present proteomic analysis of leaf tissue of Muscadine, Bunch and Florida hybrids subjected to water deficit stress. Grape plants were grown in pot culture under greenhouse conditions. Water stress was induced by withholding irrigation. The leaf samples were collected from both irrigated and stressed plants at 5, 10, 15 and 20 day interval. Proteins were extracted with trichloroacetic acid /acetone and separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). The proteins were sequenced in LC/Mass Spectrophotometer. The results indicated suppression and over-expression of low molecular weight polypeptides (10 to 60 kD) during stress in these genotypes. Fewer proteins were affected in Muscadine leaf while several proteins were suppressed in leaf tissue of bunch and Florida bunch hybrid grape upon water stress. We have found that ribulose bisphosphate carboxylase and phosphoribulokinase, which play major role in photosynthesis, were suppressed in response to water stress in Florida bunch hybrid. We have generated subtractive hybridized PCR products of water stressed transcripts in Florida bunch hybrid grape to characterize the unique genes expressed or suppressed in response to water stress. Cloning and transcript analysis are in progress in parallel with protein expression studies that may be associated with changes in nutraceutical and enological traits in grape under water deficit conditions. Such profiling studies will be used to explicate specific pathways disconcerted by water deficit treatments, and in the identification of varietal differences.


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1 - Florida A & M University, Plant Biotechnology, Center for Viticulture and Small Fruit Research, 6505 Mahan Drive, Tallahassee, Florida, 32317, USA
2 - Florida A & M University, Plant Biotechnology
3 - Mississippi State University, Mississippi State, MS 39762, Plant and Soil Science

Keywords:
Grape
Water stress
proteomics
transcripts.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P44005
Abstract ID:334


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