| Abstract Detail
Membrane Transport Gopala, Mulangi, RV [1], Phuntumart, Vipaporn [1], Morris, Paul F [1]. Characterization of a rice spermidine transporter gene by heterologous expression in yeast mutants. Polyamines are ubiquitous nitrogenous compounds found in all eukaryotic and prokaryotic cells. In plants, the levels of polyamines are correlated with the plant response to stresses such as drought, salinity and temperature. Plants readily uptake polyamines and also mobilized through the vascular tissues. However, the transport of polyamines in and out of the plant cells has not been yet linked to any plant genes. In the present study, we used a comparative genomics approach to identify and characterize polyamine transporters in rice and Arabidopsis thaliana genomes which translocate polyamines in and out of the cytoplasm. We identified three and four candidate transporter genes in A. thaliana and rice, respectively. The full-length cDNA clone of one of the rice candidate genes was transferred to the Gateway expression vector, pDEST 52, by recombination cloning. The heterologous expression of this gene in AGP2-deficient yeast mutant was mediated under the control of Gal1 promoter. Radiological time-dependent, uptake studies of the above rice gene showed an enhanced uptake of spermidine by transformants relative to both wild type and mutant strain. The kinetic characterization of this gene for spermidine and putrescine is presently underway. The future work will focus on functional analysis and phenotypic characterization of mutants of these predicted transporter genes under different environmental stress conditions.
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1 - Bowling Green State University, Biological Sciences, Life Science Building, Bowling Green, Ohio, 43403, USA
Keywords:
polyamine
transporters
rice
spermidine
phloem loading
Stress response.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P10003
Abstract ID:323 |
