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Abstract Detail


Salinity

Katz, Adriana [1], Waridel, Patrice [2], Shevchenko, Andrej [3], Pick, Uri [4].

Salt-induced changes in the plasma membrane proteome of the halotolerant alga Dunaliella salina.

The halotolerant alga Dunaliella salina responds to increasing salinity by enhancement of photosynthesis, CO2 assimilation and by diversion of carbon and energy resources for glycerol synthesis, the compatible solute. The adaptation mechanism involves major changes in the proteome composition. In order to clarify the molecular basis for the remarkable salinity tolerance, we performed a comprehensive proteomic analysis of the plasma membrane, with the aim to identify changes in the membrane protein composition and organization at high salinity. Detection of plasma membrane proteins was obtained by tagging intact cells with a membrane-impermeable biotin derivative and specific immunoreactions. Proteins were resolved by two-dimensional Blue Native/SDS-PAGE and identified by nanoLC-MS/MS. Out of 55 identified proteins, about 60% were integral membrane or membrane-associated. We identified novel surface coat proteins, lipid metabolizing enzymes, a new family of membrane proteins of unknown function, ion transporters, small GTP-binding proteins and heat shock proteins. The abundance of 20 protein spots increased and of 2 protein spots decreased under high salt. The major salt-regulated proteins included elements that function in protein and membrane structure stabilization and factors within signal transduction pathways. This comprehensive overview of a plasma membrane proteome in an extreme halotolerant organism may shed light on novel mechanisms of adaptation to high salt.


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1 - Weizmann Institute of Science, Biological chemistry, Rehovot, 76100, Israel
2 - University of Lausanne
3 - Max Planck Institute of Molecular Cell Biology and Genetics
4 - Weizmann Institute of Science

Keywords:
membrane protein
proteomics
BN gel
salinity tolerance
plasma membrane
LC-MS/MS.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P07005
Abstract ID:280


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