Unable to connect to database - 05:44:24 Unable to connect to database - 05:44:24 SQL Statement is null or not a SELECT - 05:44:24 SQL Statement is null or not a DELETE - 05:44:24 Botany & Plant Biology 2007 - Abstract Search
Unable to connect to database - 05:44:24 Unable to connect to database - 05:44:24 SQL Statement is null or not a SELECT - 05:44:24

Abstract Detail


Environmental Physiology

Takabe, Tomoko [1], Shinagawa, Fumi [2], Terada, Yasutaka [2], Uchida, Akio [3], Hibino, Takashi [4], Takabe, Teruhiro [4], Kawai, Takayoshi [5], Takabe, Tetsuko [2].

Overexpression of DnaK from a halotolerant cyanobacterium Aphanothece halophytica enhances abiotic stress tolerance of young poplar tree plants.

Previously, we have reported that overexpression of DnaK (a member of HSP 70 family) from a halotolerant cyanobacterium Aphanothece halophytica enhances tolerance to abiotic stresses such as high salinity and high temperature in tobacco plants in which DnaK protein was expressed in the cytosol. The DnaK protein was shown to exhibit extremely high protein folding activity in high salinity conditions due to its longer C-terminal segment (70-90 extra amino acid residues) than other DnaK/HSP70 family members. So it was anticipated that overexpression of DnaK might be useful to enhance abiotic stress tolerance in tree palnts (Populus alba). We used female poplar plants which are sterile under the environments where male poplar plants are absent. We obtained two lines of transgenic poplar plants, TTP1 and TTP2. It was confirmed that both transgenic lines contain DnaK protein by Western blot analysis. Here we tested the tolerance of transgenic DnaK poplar plants towards high salinity, drought and low temperature compared with those of the wild type trees. It was found that both lines of transgenic poplar plants acquire increased tolerance towards high salinity (sea water), drought (withholding water) and low temperature (-15℃). All those phenotypes were very clear in appearance and their enhanced tolerance was confirmed also by using PAM.


Log in to add this item to your schedule

1 - Nagoya University, Environmental Studies, Chikusa, Nagoya, 464-8601, Japan
2 - Nagoya University, Graduate School of Bioagricultual Sciences
3 - Nagoya University
4 - Meijo University
5 - Nagoya University, Graduate School of Environmental Studies

Keywords:
salinity
Drought
low temperature
poplar
tree .

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P01006
Abstract ID:274


Copyright © 2000-2007, Botanical Society of America. All rights