Recent Topics Posters
Oakley, Rodney , Wang, S.-Y. , Harding, Scott , Tsai, C.-J. .
alpha- and beta-Tubulin Isoforms with Distinct C-Terminal Amino Acid Residues are Differentially Expressed in Quaking Aspen.
Microtubules are involved in many cellular processes, including cell division, cell shape, intracellular transport, signal transduction and plant cell wall formation. Microtubules are composed of repeating alpha- and beta-tubulin subunits, encoded by multi-gene families with very high overall sequence homology in their protein coding regions, but divergent 5’ and 3’-UTR’s. There are eight alpha-tubulin genes present in the P. trichocarpa genome sequence, designated PtTUA1 through PtTUA8. The 8 predicted PtTUA proteins show an overall amino acid sequence identity of 88 to 98%, but are highly divergent in the last 15 amino acids. Twenty beta-tubulin genes were identified in silico from the P. trichocarpa genome sequence. These were designated PtTUB1 through PtTUB20 and their hypothetical proteins range from 89 to 98% sequence identity, also with divergent C-termini. Quantitative real-time RT-PCR results, using 3’-UTR specific primers for both PtTUA and PtTUB isotypes, showed differential expression of the gene family members across various tissue types. PtTUA1, PtTUA5, PtTUB9 and PtTUB15 transcripts are most abundant in developing xylem tissue, while PtTUA1, PtTUA6/8, PtTUB7 and PtTUB19 are preferentially expressed in mature pollens. The other tissue types showed a more uniform expression pattern of the various transcripts. Each of the alpha- and beta-tubulin isoforms may also undergo post-translational modification (PTM), all but one modification occurring at the divergent C-termini. I will discuss the expression patterns and protein levels of alpha- and beta-tubulin, andÂ PTMs of the alpha-tubulins present in quaking aspen tissues.
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1 - Samuel Roberts Noble Foundation, Plant Biology Division, 2510 Sam Noble Parkway, Ardmore, OK, 73402, USA
2 - Michigan Technological University, Biotechnological Research Center, School of Forest Resources and Environmental Sciences, 1400 Tonsend Drive, Houghton, MI, 49931, USA
Presentation Type: Recent Topics Poster
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM