| Abstract Detail
Organelle Biology Kakizaki, Tomohiro [1], Matsumura, Hideo [2], Terauchi, Ryohei [2], Inaba, Takehito [3]. Comprehensive analysis of intracellular communication between plastids and the nucleus using mutants defective in protein import receptors. Intracellular signaling between organelles is a key cellular process responding to the internal cellular environments in eukaryotic cells. In higher plants, one of the most important intracellular communications is the signal networks between the nucleus and plastids. It is generally believed that plastids evolved from a unicellular photosynthetic bacterium inside a eukaryotic host cell. At present the plastid genome encodes fewer than 100 ORFs, and most genes that were encoded by the bacterial ancestor have been moved to the host nuclear genome. This separation of genetic information necessitates the communication between plastids and the nucleus to coordinate gene expression of these genome. However the molecular mechanisms underlying this inter organelle signaling remains to be characterized. To elucidate the signal transduction pathways from plastids to the nucleus, there is significant evidence that the inhibition of protein import into plastids disrupts plastid signals. In ppi2 mutant lacking TOC159 protein import receptor, the mRNA levels of photosynthetic proteins are dramatically reduced. In this study, we performed global gene expression analysis of ppi2 mutant using SuperSAGE. We show that specific pathways that regulate certain classes of genes were affected in ppi2 mutant.
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1 - Iwate University, Cryobiosystem Research Center, Faculty of Agriculture, 3-18-8, Ueda, Morioka, Iwate, 020-8550, Japan
2 - Iwate Biotechnology Research Center, 22-174-7, Narita, Kitakami, Iwate, 024-0003, Japan
3 - The 21st Century COE Program, Iwate University, 3-18-8, Ueda, Morioka, Iwate, 020-8550, Japan
Keywords:
Chloroplast.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P18008
Abstract ID:273 |
