Roberts, Diana , Nadella, Vijayandand , Wyatt, Sarah .
Auxin Response Factor 9, a new player in gravity signal transduction?
Roberts, Diana R., Nadella, Vijayandand. Wyatt, Sarah E. Dept. of Env. Plant Biology, Ohio University, Athens, OH 45701. Gravity is a constant stimulus involved in plant growth and development. The gravity response in plants involves three basic steps; signal perception, signal transduction, and differential growth. Studies involving the gravity persistence signal (gps) mutants (Wyatt et al. 2002) have implicated the Auxin Response Factor (ARF) gene family in gravitropic signal transduction. The goal of this research was to determine the role of ARF9 in the plantís response to gravity. The expression pattern of the ARF9 transcript during the GPS treatment was studied in wild type Arabidopsis at several time points using quantitative RT-PCR. Interestingly, expression of the ARF9 transcript was increased after the GPS treatment when compared to the cold untreated. T-DNA insertion mutants for ARF9 were obtained from the SALK Institute and screened for a gravity response phenotype using the GPS treatment. One of the SALK lines with a T-DNA insertion located in exon 12, near the C terminus of ARF9, displayed increased gravitropic curvature after the GPS treatment. Semi-quantitative RT-PCR of the ARF9 T-DNA mutant indicates that the mutation could be interrupting the normal function of ARF9. These data support a role for ARF9 in gravity signal transduction. (Partially supported by Grasselli-Brown Undergraduate Research Award, Ohio University to DRR and NASA:NAG2-1608 to SEW.)
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1 - Ohio University, Environmental and Plant Biology, 508 Porter Hall, Athens, Ohio, 45701, USA
2 - Ohio University, Environmental and Plant Biology
Auxin Response Factor
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM