| Abstract Detail
Secondary Metabolism Facciotti, Daniel [1], Goodstal, F. John [2], Manning, Colleen [1], McGuire, Cate [1], Slade, Ann [1], Cochran, Michael [1]. TILLING® for isoflavone-free soybean. We are interested in evaluating the potential health risks and/or benefits of phytoestrogens in soy-foods. Here we will report on our progress in breeding for isoflavone-free (phytoestrogen-free) soybean. This project shows how TILLING® (Targeted Induced Local Lesions In Genomes) assisted breeding can be used to modify a complex trait by creating an allelic series of mutations in targeted genes. The synthesis of isoflavones involves at least two isoflavone synthase genes (IFS1 and IFS2). We have used TILLING® to select plants carrying IFS1 and IFS2 nonsense and missense mutations resulting in no, or reduced, IFS activity. We will present genotype and phenotype data for plants carrying mutations in either or both of these genes and evaluate the contribution of each IFS gene to the level of isoflavones.
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1 - Arcadia Biosciences
2 - Arcadia Biosciences, 202 Cousteau Place, Suite 200, Davis, CA, 95616
Keywords:
TILLING
isoflavone
Soybean.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P20051
Abstract ID:2703 |
