| Abstract Detail
Recent Topics Posters Kim, Sangtae [1], Wall, Kerr [2], Soltis, Douglas E. [3], Soltis, Pamela S. [4]. Phylogeny of MADS-box transcription factor gene family. MADS-box genes encode transcription factors that play important roles in the development of plants, animals, and fungi. Previous phylogenetic studies recognized two major lineages of MADS-box genes, type I and type II, and several sublineages of type II genes. However, most studies have been based on data from a limited set of taxa with biased gene sampling, a single method of analysis, and only amino acid data and consequently generated phylogenetic trees with poor resolution and low clade support. To clarify the phylogeny of MADS-box genes and their duplication history, we analyzed a large-scale data set representing genes from most major angiosperm and gymnosperm lineages. We searched for all reported MADS-box genes to date by blasting 107 Arabidopsis MADS-box genes against all GenBank data and added new genes identified from approximately 100,000 ESTs (mainly from basal angiosperm taxa) of the Floral Genome Project. With the collected 2,919 sequences, we eliminated short sequences (<70 aa), constructed contigs to identify similar sequences (99% DNA similarity level), and eliminated similar sequences based on these contigs. The final data set contained 1,244 sequences. We produced multiple alignments and analyzed them with Maximum Likelihood (using PHYML) and Bayesian inference (using MrBayes). We assessed the sister group of MADS-box genes and determined the major lineages and sublineages of MADS-box genes and their phylogenetic relationships.
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1 - University of Florida, Department of Botany, 220 Bartram Hall, P.O. Box 118526, Gainesville, Florida, 32611-8526, USA
2 - Pennsylvania State university, Dept. of Biology, Institute of Molecular Evolutionary Genetics, and Huck Institutes of Life Sciences, University Park, PA, 16802, USA
3 - University of Florida, Department of Botany, 220 Bartram Hall, P.O. Box 118526, Gainesville, FL, 32611-8526, USA
4 - University of Florida, Florida Museum of Natural History, Gainesville, FL, 32611, USA
Keywords:
mads-box
transcription factor
gene family evolution
EST
MrBayes
PHYML.
Presentation Type: Recent Topics Poster
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P79033
Abstract ID:2681 |
