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Abstract Detail


Emerging Technologies

Maiti, Indu [1], Pattanaik, Sitakanta [2], Bhattacharyya, Somnath [2], Das, Narayan [2], Raha, Sumita [2].

A novel strategy for expressing di- and tri-cistronic constructs in transgenic plants.

We have developed an expression system for constitutive expression of multiple genes from a polycistronic unit in transgenic plants. The polycistronic expression cassettes, designed for regulated expression of multiple genes, are composed of Peanut chlorotic streak virus (PClSV) full-length transcript promoter-leader and p7R (antisense orientation of PClSV ORF VII) sequence as intervening sequence; and GFP, GUS and CAT as reporter genes. The PClSV full-length transcript promoter-leader sequence and the p7R act as intron in this process, and are involved in precise splicing of the polycistronic units. It has been tested both in transient protoplasts expression experiments and in stably transformed transgenic plants. The efficient translation of polycistronic mRNAs has potential value in plant metabolic engineering.


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1 - University of Kentucky, KTRDC, Cooper & University Drives, Lexington, KY, 40546-0236, USA
2 - University of Kentucky, KTRDC

Keywords:
transgene expression
PClSV
splicing
p7R
tricistronic.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P44023
Abstract ID:2663


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