| Abstract Detail
Growth and Vegetative Development Moon, Jihyun [1], Hake, Sarah [2]. Ligulelessnarrow, a dominant mutation in maize development. Maize leaves arise from the shoot apical meristem in a distichous pattern. Downregulation of knox genes in a subset of cells lead to the formation of a leaf primordia. During growth the maize leaf develop different tissue types along the proximo-distal axis; the distal blade, proximal sheath and the auricle and ligule which form a boundary between the blade and sheath. knox genes are known to be important in the regulation of the proximo-distal patterning since when ectopically expressed in the leaves proximal tissues appear in the distal blade region. Here, we introduce a dominant mutant Ligulelessnarrow(Lgn) which also shows defects in the proximo-distal patterning. Lgn heterozygotes have disrupted blade-sheath boundaries and narrower leaves. In addition, the mutant is shorter in stature and often fail to develop ears. When homozygote for the mutation, the phenotype gets more severe. To further understand the function of the gene, double mutants were generated with previously known liguleless mutants, lg1 and lg2. The Lgn/+ and lg1 mutations synergistically affect the boundary development which lead to a complete loss of ligules and auricles in the double mutants.While Lgn/+ lg2 shows similar level of boundary defects as lg2. Using map based cloning approach, the gene has been mapped to chromosome 9.
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1 - University of California, Berkeley / Plant gene expression center, Plant and microbial biology, 800 Buchanan street, Albany, CA, 94710, USA
2 - USDA / Plant gene expression center
Keywords:
Leaf development
maize.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P26072
Abstract ID:2621 |
