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Abstract Detail


Reproductive Development

Hakozaki, Hirokazu [1], Park, Jong-In [2], Endo, Makoto [3], Takada, Yoshinobu [2], Kazama, Tomohiko [2], Takeda, Yoshimitsu [2], Suzuki, Go [4], Kawagishi-Kobayashi, Makiko [3], Watanabe, Masao [2].

Arabidopsis thaliana 3-ketoacyl-ACP synthase2 (AtKAS2) gene is required for embryogenesis.

The 3-ketoacyl-ACP synthase (KAS) enzyme creates new carbon-carbon bonds by carrying out Claisen condensation which consists of three steps. The fatty acid biosynthesis 1 (fab1) mutant of Arabidopsis thaliana is partially deficient in activity of Arabidopsis thaliana 3-ketoacyl-ACP synthase2 (AtKAS2), so we have used T-DNA insertion mutant of AtKAS2 to study possible role of the AtKAS2 in plant development. To obtain homozygous plants of the atkas2 mutant line, the isolated heterozygous plant was allowed to self-pollinate. Despite extensive screening, no homozygous mutant plants were identified by segregation analysis of progeny derived from atkas2 heterozygotes. We couldn't find atkas2 homozygous mutant adult plants in heterozygous mutant progeny. AtKAS2 gene was shown to be expressed in various plant organs except for root and highly expressed in silique. The fusion of GUS to the AtKAS2 promoter demonstrated that the promoter was active in a ovule. Phenotypic analysis showed that disruption of the AtKAS2 by T-DNA insertion caused embryo lethality and the development of the embryos was arrested at the globular stage. Taken together, our results suggest that AtKAS2 is required for embryo development in Arabidopsis during the transition from the globular to the heart stage.


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1 - Tohoku University, Graduate School of Life Sciences, 2-1-1, Katahira, Aoba-ku, Sendai, 980-8577, Japan
2 - Tohoku University, Graduate School of Life Sciences
3 - National Institute of Crop Science, Laboratory of Biotechnology
4 - Osaka Kyoiku University, Division of Natural Science

Keywords:
Arabidopsis thaliana
embryogenesis
3-ketoacyl-ACP synthase.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P28061
Abstract ID:2588


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