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Abstract Detail


Education & Outreach

Tsay, Hsin-Sheng [1], Chen, Chin-Fun [1], Chen, Chien-Fu [2].

Activation tagging approach in a medical fungi, Antrodia cinnamomea.

In order to introduce concepts about activation tagging and Agrobacterium-based transformation, we used Antrodia cinnamomea, a Taiwanese medical fungi, to develop a laboratory exercise. Agrobacterium tumefaciens strain EHA105 harboring a standard binary vector pTAG-8 was used to generate transgenic mycelia population. More than 50 transgenic lines with normal and abnormal phenotypes were obtained. After HPLC and phenol-sulfuric acid analysis, the transgenic lines showed different compound profiles. Through this laboratory exercise, students learned not only knowledge of biochemical analysis, but also molecular biology techniques.


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1 - Chaoyang University of Technology, Graduate Institute of Biotechnology
2 - Chaoyang University of Technology, Graduate Institute of Biotechnology, 168, Gifong E. Rd., Wufong Township, Taichung County, TAIWAN, 41349, TAIWAN

Keywords:
Activation Tagging
Antrodia cinnamomea.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P46027
Abstract ID:2579


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