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Abstract Detail


Environmental Physiology

Rathinasabapathi, Bala [1], Wu, Shan [2], Sundaram, Sabarinath [2].

Enhanced tolerance to high temperature stress in transgenic tomato expressing bacterial panD gene.

Tomato Cv. Early Girl and Cv. MicroTom were transformed using Agrobacterium tumefaciens-mediated transformation with a construct wherein panD gene of Escherichia coli was cloned under a constitutive plant promoter. Putative transgenic plants, identified by their tolerance to kanamycin, were screened for the presence of the transgene using a polymerase chain reaction procedure. Expression of panD transgene was analyzed using a quantitative RT-PCR technique. Seedlings of two homozygous panD transgenic lines of Cv. Early Girl background, were subjected to an evaluation of their thermotolerance. Five week-old plants were exposed to 400C for one week in a growth chamber and returned to recover for a week at a greenhouse with average maximum and minimum temperatures of 350C and 200C respectively. The two lines expressing the panD transgene were significantly more tolerant to high temperature stress than control plants based on phenotypic observations after heat stress and quantitative data on biomass yield per plant after recovery from stress. This work was supported by a grant from the USDA (2005-34135-15898).


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1 - University of Florida, Horticultural Sciences Department, Hull Rd, University of Florida, Fifield Hall, Gainesville, FL, 32611-0690, U.S.A.
2 - University of Florida, Gainesville, Horticultural Sciences Department

Keywords:
stress resistance.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P01053
Abstract ID:2576


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