| Abstract Detail
Recent Topics Posters Feliciano, Natalie M. [1], Miller, Jill S. [2]. Highly similar S-RNase alleles among Lycium (Solanaceae) species. Self-fertilization is avoided in gametophytic self-incompatible plants through the interaction of two genes: SLF (expressed in haploid pollen) and S-RNase (expressed in diploid styles). In Solanaceae, the S-RNase gene is composed of five conserved and two hypervariable regions and contains a single intron. Recent S-RNase surveys coupled with controlled pollinations of African Lycium suggest that self-incompatibility has persisted despite a single long-distance dispersal from the Americas to the Old World. Surveys of African S-RNase sequences have also revealed identical S-RNase partial alleles (ca. 380 bp) in several different species pairs. To confirm the presence of identical S-RNases in different species and to compare intron sequence diversity, I designed allele-specific primers and amplified the putatively shared allele in pairs of species using genomic DNA as a template. Preliminary results indicate that several species pairs of Lycium share identical sequences, including identical intron sequences. I also investigated putatively shared alleles in North American Lycium and have confirmed the presence of an identical partial allele and intron in 2 species pairs. The radiation of African Lycium is hypothesized to have occurred quite recently; thus it is possible that different species share S-RNase alleles retained through speciation events. Alternatively, gene flow between extant species or recent hybridization may explain S-RNase allele sharing. Further analyses of S-RNase sequences to investigate these possibilities are ongoing and will determine the extent of S-RNase allele sharing among species of Lycium.
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1 - University of Massachusetts, Amherst, Plant Biology Graduate Program, 217 Morrill Science Center South, 611 North Pleasant Street, Amherst, MA, 01003, United States
2 - Amherst College, Department of Biology, Mcguire Life Sciences Building, Amherst, Massachusetts, 01002, USA
Keywords:
self-incompatibility
S-RNase
Lycium
intron
Solanaceae.
Presentation Type: Recent Topics Poster
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P79012
Abstract ID:2571 |
