| Abstract Detail
Plant-Pathogen Interactions Fraire-Velázquez, Saul [1], Pescador-Flores, Blanca [2], Rodríguez-Guerra, Raul [3], Rodríguez-Kessler, Margarita [4], Jiménez-Bremont, Francisco [4], Simpson-Williamson, June [5]. Biocontrol of root pathogens and gene induction in seedlings of Capsicum annuum with native isolates of binucleate Rhizoctonia. In Capsicum annuum, alternative strategies for to the control of damping-off diseases caused by the pathogens Phytophthora capsici, Rhizoctonia solani, Fusarium oxysporum, F. solani and other fungus is using biocontrol agents, among these, avirulent strains of binucleate Rhizoctonia. The protecting effect in plant could include the activation of the systemic acquired resistance (SAR), a physiological state of enhanced defense capacity. Few investigations have focused on the molecular bases of plant responses to binucleate Rhizoctonia. In this study we focused on the isolation of native fungal specie, colonizers of root of chili pepper and common beans grown in fields of Zacatecas and Guanajuato Mexican provinces, to obtain virulent R. solani and avirulent binucleate Rhizoctonia isolates. With the more promising biocontrol agent of binucleate Rhizoctonia against F. oxysporum and F. solani pathogens in Capsicum annuum seedlings, the searching of plant genes induced during early root colonization by the selected avirulent strain, by means of suppressive subtractive hybridization protocol. One hundred fifty four isolates of Rhizoctonia solani were collected, forty nine belonged to binucleate and 105 to multinucleate. Two strains of binucleate Rhizoctonia (one from chili and other from common beans) protected against Fusarium spp. and P. capsici, and differentially against R. solani. From the suppressive subtractive hybridization, in plant in interaction with one of the avirulent isolates and control plant, in 130 sequenced clones, 16 % corresponds to genes implicated in signal transduction, 10 % in metabolism, 13 % in control of gene expression, 24 % are defense genes, 20 % unknown proteins, and 16.5 % pertain to the fungus. The data suggest the activation of SAR in plant.
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1 - Universidad Autónoma de Zacatecas, Unidad Ac. Biología Experimental, Dept. Biología Molecular de Plantas, Av. Revolución S/N, Col. Tierra y libertad, Guadalupe, Zacatecas, 98600, México
2 - Universidad Autónoma de Zacatecas, Unidad Ac. Biología Experimental
3 - INIFAP-Campo Experimental Bajío
4 - Instituto Potosino de Investigación Científica y Tecnológica (IPICyT), División de Biología Molecular
5 - CINVESTAV-IPN, Campus Guanajuato, Ingeniería genética
Keywords:
binucleate Rhizoctonia
SAR induction
Capsicum annuum
plant defense.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P15091
Abstract ID:2487 |
