| Abstract Detail
Growth and Vegetative Development Behbahani, Mandana [1]. Establishment of Barringtonia racemosa regeneration from leaf explants. Abstract Plantlet regeneration through shoot formation from young leaf explant-derived callus of Barringtonia racemosa is described. Calli were aseptically obtained by placing surface sterilized leaf explants on Woody Plant Medium (WPM) supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D). On the shoot induction medium, the callus induced on the WPM medium containing 2 mg/L (w/v) KIN+0.2 mg/l (w/v) IBA and 2 mg/L (w/v) of KIN +0.4 mg/L(w/v) of NAA was the most effective, providing high shoot regeneration frequency of 85.6 and 76.5 % respectively. In addition, the highest number of shoots produced was 8.2 and 6.3 shoots per explant respectively in the medium containing the mentioned phytohormones. The good rooting percentage 62 and 5.6 % roots per shoot were achieved on WPM medium supplemented with 3g/L (w/v) of active charcoal and 0.8 mg/L (w/v) of IBA. Approximately 96 % of the in vitro rooted plantlets with well developed shoots and roots were survived transferring to mixed soil and grown well in the glass house.
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1 - malaysia, selangor, serdang, UPM
Keywords:
none specified
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P26069
Abstract ID:2481 |
