Yeun, Li Huey , Messinese, Elsa , Mun, Jeong-Hwan , Jayaraman, Dhileepkumar , Lougnon, Geraldine , Rouge, Pierre , Barre, Annick , Bono, Jean-Jacques , Cook, Douglas R. , Ane, Jean-Michel .
A novel nuclear protein interacts with the symbiotic DMI3 CCaMK of Medicago truncatula.
Most higher plants are able to develop symbiotic associations with arbuscular mycorrhizal (AM) fungi that facilitate the uptake of inorganic nutrients from the soil and protects them against biotic and abiotic stresses. Legumes can also enter into a mutualism with nitrogen-fixing bacteria named rhizobia. Several genes that control such interactions have been cloned in model legumes using forward genetic approaches. DMI3 (Does Not Make Infections 3) encodes a calcium/calmodulin dependent kinase (CCaMK) that is required for the establishment of both legume nodulation and AM symbiosis. We are interested in identifying proteins that interact with DMI3 either as controlling or as targets of its kinase activity. We employed a yeast two-hybrid approach and identified a novel protein that interacts with DMI3 named IPD3 (Interacting Protein of DMI3). IPD3 is predicted to interact with DMI3 through a C-terminal coiled-coil domain. Like DMI3, this protein was shown to be localized to the nucleus of Medicago truncatula root cells. Split yellow fluorescent protein (YFP) assays suggest that DMI3 interacts physically with IPD3 in the nuclear compartment. Promoter fusion to the uidA (GUS) gene shows that IPD3 expression increases in the cortex prior to nodule formation. Both IPD3 and DMI3 are well conserved among the angiosperms with the notable exception of Arabidopsis thaliana. Progress towards the characterization of this novel protein will be reported.
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1 - University of Wisconsin, Department of Agronomy, 1575 Linden Drive, Madison, Wi, 53706, USA
2 - CNRS-UPS Toulouse III, UMR 5546
3 - University of California, Department of Plant Pathology
4 - University of Wisconsin, Department of Agronomy
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM