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Abstract Detail


Plant-Pathogen Interactions

Bartley, Laura [1], Peng, YIng [2], Jung, Kihong [3], Chern, Mawsheng [3], Dardick, Christopher [4], Ruan, Randy [3], Ronald, Pamela [3].

The Role in Disease Resistance and Mechanism of Action of XB10, a Putative WRKY-Family Transcription Factor that Interacts with the XA21 Rice Pathogen Recognition Receptor.

Xa21 confers rice with resistance to bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo), and codes for a protein with an extracellular leucine-rich repeat domain and an intracellular kinase. Plants possessing Xa21 perceive bacteria that secrete a specific factor, AvrXa21, into the environment. Recognition triggers a signaling cascade leading to altered gene expression and disease resistance; however, few of the signaling molecules have yet been characterized. To identify such proteins, we conducted yeast two-hybrid screening of rice cDNA libraries for proteins that interact with XA21. One of eight XA21 binding (XB) proteins identified in this screen was XB10, a member of the WRKY zinc finger transcription factor family. Consistent with a functional interaction between XB10 and XA21, RNA microarray analysis shows that levels of Xb10 transcript increase upon inoculation of Xa21-expressing plants with Xoo. Additional screening with XB10 as bait identified three other WRKY IIa members that interact with XB10, termed XB10i-1, -2, and -3. Simultaneous over expression of all four of the WRKY IIa genes leads to a partial resistance phenotype. As we do not know the native DNA sequence to which XB10 binds, we have constructed a chimeric protein in which the putative DNA binding domain of XB10 is replaced with the DNA binding domain of the yeast GAL4 transcriptional activator. We can use the chimera to determine if and under what conditions XB10 effects transcription. Under un-induced conditions, the XB10-GAL4 chimera does not induce transcription of a luciferase construct with a promoter that contains the GAL4 up-stream activating sequence; whereas, a control chimera made from the viral transcriptional activator, VP16, induces high levels of luciferase expression.


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Related Links:
Ronald Lab Website


1 - University of California Davis, Plant Pathology, Genome Center, 5212A, One Shields Avenue, Davis, CA, 95616, United States
2 - University of California Davis, Genetics
3 - University of California Davis, Plant Pathology
4 - Agricultural Research Service, Appalachian Fruit Research Station

Keywords:
rice
Signal transduction
bacterial defense
kinase.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P15014
Abstract ID:243


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