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Abstract Detail


Chromatin

Zhu, Yan [3], Dong, Aiwu [1], Meyer, Denise [3], Renou, Jean-Pierre [2], Shen, Wen-Hui [3].

NRP1 and NRP2 histone-chaperones are required for maintaining postembryonic root growth.

NUCLEOSOME ASSEMBLY PROTEIN 1 (NAP1) is conserved from yeast to human and was proposed to act as a histone chaperone. While budding yeast contains a single NAP1 gene, multicellular organisms including plants and animals contain several NAP1 and NAP1-RELATED PROTEIN (NRP) genes. However, the biological role of these genes had been largely unexamined. Recently, we reported that, in Arabidopsis, simultaneous knockout of the two NRP genes, NRP1 and NRP2, impaired post-embryonic root growth (Zhu et al., Plant Cell 18: 2879-2892). In the nrp1-1nrp2-1 double mutant, arrest of cell cycle progression at G2/M and disordered cellular organization occurred in root tips. The mutant seedlings exhibit perturbed expression of about a hundred genes, including some genes involved in root proliferation and patterning. The mutant plants show higher sensitive to genotoxic stress, increased levels of DNA damage and the release of transcriptional gene silencing. NRP1 and NRP2 are localized in the nucleus and can form homo- and heteromeric protein complexes. Both proteins specifically bind histones H2A and H2B and associate with chromatin in vivo. We propose that NRP1 and NRP2 act as H2A/H2B chaperones in the maintenance of dynamic chromatin in epigenetic inheritance.


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1 - Fudan University, Biochemistry, Shanghai, 200433, PR China
2 - URGV, 2 rue Gaston Crémieux, Evry, 91057, France
3 - IBMP-CNRS, Cell Biology, 12 rue du General Zimmer, Strasbourg, 67084, France

Keywords:
histone
Chaperone
chromatin-remodeling
Gene Silencing
root growth
DNA damage.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P39006
Abstract ID:2396


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