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Abstract Detail


Seed Biology

Shen-Miller, J. [1], Xie, Y. [2], Wooding, K. [3], Yang, Y. [3], Villa, S. [4], Zhang, H. [3], Lindner, P. [5], Loo, J. [3], Clarke, S. [4].

Exceptional longevity of fruits of Nelumbo nucifera: heat-hardy (100oC) proteins.

Fruits (hard pericarp-containing seeds) of Nelumbo nucifera (sacred lotus) are the longest living (1300 y) known in plants. Nelumbo seed tissues contain abundant hardy proteins that remain soluble to high temperature (110oC). The total content and heat stability of seed proteins of a 549-y-old fruit are indistinguishable from those of modern controls. Nelumbo chaperonins are more abundant and more heat stable than those of French beans and wheat. At >80oC, the solubility of high molecular-weight proteins (>78 kDa) decreases in beans and wheat (half-life seed longevity ~8-16 y) but remains appreciable in old and modern Nelumbo. Heat-stable proteins (100oC) of Nelumbo embryo-axes, cotyledons and pericarp* by peptide sequencing (nano-LC, tandem-MS), Western blots** and bioassy^ show them to share sequence homology or protein identity with the following proteins of plants (kDa corresponding to those of protein standards on SDS-PAGE gels of Nelumbo, or Mr): ~200 kDa, glycine dehydrogenase/ADP-ribose polymerase; ~100 kDa, nucleolin; ~85 kDa, cobalamin-independent methionine synthase/Hsp 80/RNA-binding protein; ~50 kDa, EF 1-alpha/Ser-Thr kinase/RNA-binding protein/vicillin/enolase; ~37 kDa, actin/vicillin precursor/aldose reductase/Pi-ta protein; ~25 kDa, seed-maturation protein/dehydrin**/triosephosphate isomerase/chaperonin cpn20**/Cys peroxiredoxin/glutathione transferase/L-isoaspartyl methyltransferase^; ~15 kDa, Cu-Zn superoxide dismutase/Hsp 18*/cyclophilin 2*/translation-controlled tumor protein homolog (TCTP)*/ADP ribosylation factor*. Some of the proteins noted above are necessity- and stress-related species whose possible functions (cf. rescue, defense, structure, storage reserve, signal transduction, antioxidative response, repair, etc.) in the long-term maintenance of seed viability will be discussed.


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1 - University California, Los Angeles, Inst Geophys Planetary Phys-Ctr Stdy Evol Orig Life; Dept Ecol, Evol Biol, 5687 Geol. Bldg., Hilgard Ave., Los Angeles, CA, 90095-1567, USA
2 - UCLA, Chem/Biochm
3 - UCLA, Chem/Biochem
4 - UCLA, Mol Biol Inst
5 - Univ Regensburg, Inst Microbiol

Keywords:
longest-living fruits
Nelumbo nucifera
Heat-stable proteins
defence- and repair-related proteins.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P29004
Abstract ID:237


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