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Abstract Detail


Education & Outreach

Robertson, Amber L. [1], Phillips, Allison [2].

Interactive approach to teaching PCR theory and primer design using bioinformatics and problem-based learning.

Polymerase chain reaction (PCR) is a conceptually difficult technique that embodies many fundamental biological processes. Traditionally, students have struggled to analyze PCR results due to an incomplete understanding of the biological concepts (theory) behind DNA replication and complementarity. We developed an interactive primer design exercise using the principles of scientific teaching to enhance student understanding of theory and provide practice in designing biological reagents. Students designed primers to amplify a portion of the aroA gene in the context of PCR. Putting the exercise in this context highlighted the importance of understanding the theory behind PCR, and in the end, the students were more poised to troubleshoot problems that arose in real experiments using PCR. In addition, students had the opportunity to utilize several bioinformatics tools that are publicly available on the World Wide Web. The students gained an increased understanding of primer quality, orientation, and specificity. In the course of this study many misconceptions about DNA replication and the need for primer specificity were identified and addressed.


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1 - University of Wisconsin- Madison, Horticulture, 1575 Linden Dr., Madison, WI, 53706, USA
2 - University of Wisconsin- Madison, Genetics

Keywords:
scientific teaching
primer design
PCR
bioinformatics.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P46005
Abstract ID:235


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