Economic Botany: Applied Plant Biology
Mathur, Meeta .
Guggulsterone production in cell suspension cultures of a tree, Commiphora wightii grown in shake flasks and bioreactor.
Commiphora wightii (Arnott.) Bhandari (syn. Commiphora mukul, Burseraceae) is a slow growing balsamiferous medicinal tree indigenous to Indian subcontinent. Traditionally its gum resin is used in arthritis, join pain, obesity and acne. Its hypocholesterolemic, and hypolipidemic activity is due to presence of guggulsterones –E and –Z in the gum resin. Biotechnological methods are used for its micropropagation and production of guggulsterone in cell cultures. The callus and cell suspension cultures derived from zygotic embryos and leaf explants were exploited for the optimal production of these bioactive molecules. Cell cultures were established from the callus and conditions for growth and guggulsterone production have been optimized. A two-stage culture system has been developed for optimal growth and guggulsterone production. Maximal cell growth and guggulsterone production was achieved using an inoculum density of 10% (v/v). The cell cultures were grown in growth medium as well as in production medium for scale-up biomass production in 250, 500ml, 1L, 2L and 3L flasks and 2L stirred tank bioreactor and 6.5L airlift bioreactor. Transferring cells in the ‘production medium’ had resulted in improved guggulsterone accumulation. A combination of sucrose: glucose (20:20g/l) in the medium was recorded optimal for guggulsterone accumulation over single sugar in same or different concentrations. Further, a combination of total 4% sucrose: glucose, precursors (phenylalanine, pyruvic acid, xylose, sodium acetate), and replacement of 2, 4-D and kinetin with morphactin and 2iP resulted in marked increase in guggulsterone accumulation and yield per litre medium. Biomass and guggulsterone yield was high in large vessels and 2L stirred tank bioreactor (67 mcg/l) (~200 mcg/l) as compared to 250ml flasks.
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1 - M. L. Sukhadia University, Department of Botany, Laboratory of Bio-Molecular Technology, Udaipur, 313001, India
Presentation Type: Oral Paper:Papers for Topics
Location: Lake Huron/Hilton
Date: Monday, July 9th, 2007
Time: 10:45 AM