Plant biotech & Risk Assessment
Loganathan, Murugan , Maruthasalam, Subbiyan , Yu, Chih-Wen , Hsu, Wen-Hwei , Lin, Chin-Ho .
A new system of soybean regeneration through somatic embryogenesis using immature embryonic tips.
Soybean (Glycine max L) is an important source of vegetable proteins because of their high nutritional values and human health factors. Improvement of soybean seed protein qualities through genetic engineering with seed-specific expression of functional peptides has been the primary objective of scientists worldwide, which depends largely on availability of a reliable and reproducible system of plant regeneration. Here we report an efficient system of soybean cv. Kaohsiung 10 (a popular cultivar in Taiwan) regeneration through somatic embryogenesis by using immature embryonic tips (excised from immature pods harvested 2-3 weeks after flowering) as explants. Primary somatic embryos were initiated from embryonic tips upon continued culturing for 3 months on MS medium supplemented with high concentration of 2.4-D while, secondary somatic embryos were induced 1-2 month after culturing on the same medium with low concentration of 2.4-D. The secondary somatic embryos took one month to develop into cotyledonary stage embryos on hormone-free MS basal salts. The embryos were subsequently desiccated for 4-5 d in sterile Petri dishes followed by plant regeneration on hormone-free MS medium. The regenerated plants were transferred onto Yoshida’s culture solution for hardening. The response of various explants, both mature seed- derived (cotyledon, embryonic tip, leaf, shoot and root) and immature seed-derived (cotyledon and embryonic tip) explants to undergo somatic embryogenesis are discussed. The protocol thus developed is being employed in Agrobacterium-mediated transformation of soybean with a view to improving the nutritional qualities of seed protein by seed-specific expression of a modified ammonium transporter (amt1-M3) gene.
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1 - National Chung-Hsing University, Department of life Sciences, Taichung, Taiwan
2 - Department of Molecular Biotechnology, Da Yeh University, Chang Hua, Taiwan
3 - National Chung-Hsing University, Institute of Molecular Biology, Taichung, Taiwan
Immature embryonic tip
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM