Systematics Section / ASPT
Madriñan, Santiago , Chacon, Juliana .
Specimen identification through DNA barcodes in the Lauraceae.
The Lauraceae are a major component of tropical tree floras. Worldwide they comprise 52 genera and ca. 2,750 species. Lauraceae are notorious among field botanists who consider identification of genera and species as extremely difficult. One of the striking characteristics of Lauraceae is that species almost identical in leaf characters can differ profoundly in floral or fruiting morphology, both within and between genera. Another serious problem in identifying Lauraceae is that vegetative characters often vary considerably within species, populations, and even individuals. Different collections of the same species have been described in different genera. Recent studies of molecular variation within Lauraceae geared towards a better understanding of generic delimitation and relationships, coupled with the efforts in establishing species identification through DNA bar-coding, may prove to be the salvation of what Alwyn Gentry once referred to as “…the taxonomic chaos that prevails in the Lauraceae”. As part of a multi institutional project geared towards the establishment of a DNA barcode for land plants, we sequenced the tentative barcode chloroplast DNA regions (accD, matK, ndhJ, rpoB, rpoC1 and trnH- psbA) for 58 specimens from 43 species in 17 genera in the Lauraceae. Ease of sequence alignment and nucleotide variation were scored for each region. Identification matches using BLAST with available GenBank sequences were tried for each specimen with high success rate.
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"Establishing a DNA barcode for land plants" project
Laboratorio de Botanica y Sistematica
1 - Universidad de los Andes, Departamento de Ciencias Biologicas, Laboratorio de Botanica y Sistematica, Apartado Aereo 4976, Bogota, DC, 00001, Colombia
DNA Barcode Markers
DNA Bar coding
Presentation Type: Oral Paper:Papers for Sections
Location: Continental C/Hilton
Date: Tuesday, July 10th, 2007
Time: 11:30 AM