| Abstract Detail
Organelle Biology Wyman, Aaron J. [1], Klanrit, Preekamol [2], Manalo, John P. M. [3], Ali, Ahson [4], Walker, Sheryl A. [2], Anderson, Carrie M. [2], Webb, Mary Alice [2]. Isolation and Characterization of Proteins Associating with Calcium Oxalate Crystals. The process of biomineralization has become increasingly important in fields ranging from materials sciences to medicine. However, many factors controlling biomineralization in vivo remain uncharacterized. The Webb lab is working on further elucidating calcium oxalate (CaOx) crystal formation in several eukaryotic systems, including plants. Plants [including grape (V. labrusca)] are hypothesized to form CaOx crystals for several reasons, including protection against herbivory, heavy metal detoxification, and as a mechanism to remove excess cytosolic calcium. Previous work on organisms that form calcium biominerals has implicated both inter and intra-crystalline proteins as having roles in controlling crystal growth and morphology. Similarly, the Webb lab has identified and investigated proteins associating with needle-shaped CaOx crystals (raphides) in grape. Among these raphide-associated proteins (RAPs) is a homolog of mammalian cochaperone Hsp-70 interacting protein (Hip). We present characterizations of the structure and function of several plant Hip orthologs. To identify more grape RAPs, we have utilized proteomics approaches. Both inter and intra-crystalline associating proteins were extracted, separated by SDS-PAGE, and analyzed by MALDI-MS to identify their peptide sequences. BLAST searches based on these amino acid sequences were conducted to identify possible eukaryotic homologs. We are cloning and expressing grape DNA sequences encoding for identified homologous proteins to investigate their polypeptides’ effects on CaOx crystal development in vitro. It is hoped these findings will further elucidate CaOx formation in vivo and potentially aid development of methods for modifying the growth of human CaOx kidney stones.
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1 - Purdue University, Botany and Plant Pathology, B035 Whistler Hall, 170 South University, West Lafayette, IN, 47907, USA
2 - Purdue University, Botany and Plant Pathology
3 - Wabash College, Biology, P.O. Box 352, Crawfordsville, IN, 47933, USA
4 - Wabash College, Chemistry, P.O. Box 352, Crawfordsville, IN, 47933, USA
Keywords:
Calcium
oxalate
biomineralization
crystal
raphide
proteins
Hip.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P18006
Abstract ID:232 |
