| Abstract Detail
Temperature Responses Hunag, Bin [1], Chen, Yih-Ming [2], Chen, Shu-Ling [3]. Transcriptome and Proteome Changes of Brassinsoteroid biosynthetic gene and Down-stream Proteins under Chilling Condition in Mung Bean Seedlings. Mung bean CYP90A2 is a putative brassinosteroid (BR) synthetic gene that shares 77% identity with the Arabidopsis CPD gene. The transcriptome of this gene includes genes that are: (1) abundantly detected in leaves that increase following seedlings development, (2) exhibit diurnal variation, (3) feedback regulated in the existence of BR, and (4) strongly suppressed by 10℃ chilling stress. With the exogenous treatment of BR, chilling-inhibited growth and survival rate could be improved. Surveying the BR-promoted epicotyl elongation against chilling stress by means of proteomics, several proteomes are revealed. One of them, BR up-regulates 31 and down-regulates 24 proteins under normal growth temperature. In another, chilling stress up-regulates 5 and down-regulates 24 proteins. Finally, there are 17 chilling stress down-regulated proteins exhibit re-up-regulation after BR treatment. Identification of these proteins using mass spectrometry (MALDI-Q-TOF MS/MS) suggests that most are involved in cell growth, photosynthesis and respiration. In the physiological assays with reference to these proteomes, two new findings are proposed. First, BR promotes epicotyl elongation only under high light condition, and secondly, chilling-inhibited mung bean epicotyl elongation could be partially recovered by exogenous treatment of DL-methionine. In summary, this is the first proteomic mapping established in mung bean, and the regulation between brassinosteroid and chilling response is investigated, to which, possible mechanisms are revealed.
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1 - Institute of Plant Biology, National Taiwan University
2 - Institute of Plant Biology, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Institute of Plant Biology, National Taiwan Univer, Taipei, 10617, Taiwan
3 - Department of Biotechnology, Ming Chuan University
Keywords:
low temperature stress
gene expression level
biotic stress.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P08030
Abstract ID:2312 |
