| Abstract Detail
Mechanisms of Gene Regulation Ben-Ghaly, Labeed [1], Gamboa, Jeanne Dyan [1], Hsiung, Jamie [1], Reyes-Izquierdo, Tania [1], Vellanoweth, PhD, Robert L. [1]. Protein Interaction at LTP Module in Arabidopsis thaliana During Bolting Transition. Lipid-based signaling pathways are known to control responses to wounding, pathogen infection, and also appear to coordinate developmental events, including the fundamental transition from vegetative to reproductive growth in plants. Two extracellular lipid transfer protein genes are significantly up-regulated in leaves as the apical meristem switches to floral growth in Arabidopsis thaliana. A highly conserved, potential cis-regulatory element (the LTP Module) located in the promoters of these LTP genes, as well as in a homologous LTP gene in Brassica napus, was synthesized and used in electrophoretic mobility shift assays (EMSA) using nuclear extracts prepared from pre-bolt and short-bolt leaves. We found that changes in protein binding of this element coincide with light induction of this transition. Results from real time RT-PCR also demonstrate that light induction of reproductive growth increases LTP mRNA in meristems and leaves. The EMSA results indicate that the newly identified nuclear proteins that bind to the LTP Module may be involved in this induction. An in vivo approach has been implemented to characterize the 100-base pair LTP Module of these genes. We want to be able to determine what happens to gene expression during the floral transition if the module gets deleted or mutated. So far, the wild-type promoter has been fused to the luciferase gene via PCR fusion, cloned into the pART27 vector and transformed into Arabidopsis thaliana. We will next quantify LTP luciferase activity through transient expression assays and in transgenic plants.
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1 - California State University Los Angeles, Chemistry/Biochemistry
Keywords:
lipid transfer protein
cis-regulatory element
lipid signaling
promoter
LTP
Luciferase.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P36051
Abstract ID:2256 |
