Lema, Alice-Patience Valentino , Ewy, Robert G. .
APX1 Expression Patterns in Soybeans Grown Under Elevated Ozone and CO2 Conditions.
The composition of our atmosphere is changing. Carbon dioxide levels in the troposphere are expected to steadily increase ca. 50% from present levels in less than fifty years. The amount of ozone is also expected to increase over this time. Most agricultural regions in the United States are exposed to elevated levels of ozone sometime during the growing season. Previous work showed that increased ozone levels have detrimental effects on many crop plants. Soybeans (Glycine max) are especially sensitive to elevated levels of ozone and show significant yield loss when continuously exposed to above ambient levels of ozone. Unlike CO2, trophospheric levels of ozone fluctuate daily so that soybeans are exposed to varying levels of this ROS throughout the growing season. We are particularly interested in understanding how increasing levels of tropospheric ozone effect soybeans. To this end, we measured the gene expression levels of Ascorbate Peroxidase (APX1), an enzyme involved in the antioxidant defense system, in soybean plants exposed to elevated ozone (20% above ambient), elevated CO2 (550 ppm), both elevated ozone and CO2, or ambient conditions. Soybeans were grown under field conditions at the SoyFACE field site in Champaign, Illinois. Leaf samples were collected five times throughout the growing season. We used Real-time PCR to determine expression patterns of the APX1 gene over the 2006 growing season. Ozone only-treated plants showed a slight decrease in APX1 expression after stage. Those plants exposed to both elevated CO2 and elevated levels of ozone showed negligible changes in APX1 expression compared to the housekeeping gene ubiquitin. This work was supported by an HHMI summer undergraduate research award to APVL (No. 52005130).
Log in to add this item to your schedule
1 - Knox College, Biology
2 - SUNY Potsdam, Biology, 44 Pierrepont Ave, Potsdam, New York, 13676, USA
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM