Systematics Section / ASPT
Lee, Hae-Lim , Kim, Jeong-Seong , Yi, Dong-Keun , Kim, Heong-Tae , Yang, So-Yeong , Weon, Kyeong-Hwa , Kim, Yeong-Kyu , Kim, Ki-Joong .
Phylogeny of Panax(Araliaceae) based on the complete chloroplast DNA sequences and the development of DNA barcoding makers at species level.
Complete chloroplast DNA sequences were generated from seven species of Panax, one species of Aralia and a species of Eleuthrococcus (Araliaceae). We reconstruct the species phylogeny using both coding and noncoding regions of chloroplast DNA. Several variable regions, that they recover fully resolved species phylogeny compare to full sequence tree, were identified to be good candidate makers for the DNA barcoding at species level. These regions also have more than one autamorphic base substitutions for all species. We also apply the DNA barcoding markers to 10 other genera of flowering plants including Syringa(Oleaceae), Taraxacum(Asteraceae), Eleuthrococcus(Araliaceae), Cardamine(Brassicaceae) and Carex(Cyperaceae), etc. Comparative analysis of the Panax chloroplast sequences reveals several small inversions both in the intergenic spacer and the intron regions. We identified 32 simple sequence repeats (defined by more than 10 times repeats of same nucleotide) from diverse regions of the genome. The absence and presence of repeats also identified all species of Panax. Most gene coding regions show low levels of Ka/Ks ratios because of strong purifying selection, however, relative high levels of Ka/Ks ratios were observed from ycf2, clpP, ycf1, accD, and matK genes.
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1 - Korea University, School of Life Sciences and Biotechnology, Anamdong-5-1, Seongbukgu, Seoul, Seoul, 136-701, Korea
complete plastome sequence
DNA Barcode Markers.
Presentation Type: Poster:Posters for Sections
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM